%0 Journal Article
%T APPLICATION OF NONLINEAR REGRESSION MODEL TO DETECTTHE THICKNESS OF PROTEIN LAYER<br>非线性模型在蛋白质薄膜厚度测定中的应用
%A WANG De-yu
%A JIANG Du-xiao
%A CHENG Shu-jian
%A ZHENG Guo-liang
%A YUAN Chun-wei
%A <br>王德育
%A 蒋笃孝
%A 陈树建
%A 甄国亮
%A 袁春伟
%J 生物物理学报
%D 2000
%I 
%X The thickness of protein layer was studied by applying nonlinear regression model to fit the reflection spectrum.To reduce the influence of systematic noise, a polystyrene film was spread on the slide surface. The reflection spectrum was normalized to that of bare glass slide substrate to eliminate the light source influence. The results were compared with that of the classical interferometry and enzyme labeled antigen-antibody interactions. Good agreements among these results suggested the proposed method is authentic.
%K Thin film
%K Nonlinear regression model
%K Antigen-Antibody interaction
%K White light interference biosensor<br>光学干涉
%K 非线性回归模型
%K 蛋白质
%K 薄膜厚度测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=5BD8785726A3EA73&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=CA4FD0336C81A37A&sid=27746BCEEE58E9DC&eid=16D8618C6164A3ED&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=16