%0 Journal Article
%T GATING KINETICS OF SINGLE POTASSIUM CHANNEL OF CULTURED RAT CEREBRAL NEURON<br>大鼠大脑皮层神经元单离子钾通道门控动力学
%A Chen Jian Liu Keqiu Liu Jinrong Hao Xuemei Fang Li
%A <br>陈健
%A 刘克球
%A 刘锦荣
%A 郝雪梅
%A 方黎
%J 生物物理学报
%D 1992
%I 
%X Single potassium channel current of cultured rat cerebral neuron were recorded with cell-attached and inside-out patch clamp. The mixed kinetic parameters and the transition rates of aggregative conformation states were identified by residual error and nonlinear least square method. The kinetic characteristics of cell-attached patch on cultured cerebral neuron of new-born rat was different from that of inside-out patch at holding potential-40 mV with symmetric potassium solution in bath and pipette. The open time probability distribution function obeyed one state distribution and the mean open-time in cell-attached mode was 2.53 ms while in inside-out mode was 2.04 ms. The closed-time probability distribution function obeyed three states distribution and the mean closed-time was 3.36 ms in cell-attached mode but was 7.58 ms in inside-out mode. The transition rates of the four states were identified by nonlinear least square method. The channel mainly sojourned at the first and the second closed state in cell-attached mode, but mainly sojourned at the first closed state in inside-out mode duriog closed period.
%K Patch clamp
%K Ion channel kinetics
%K Potassium channel
%K Neuron
%K System identification<br>膜片钳
%K 通道动力学
%K 神经元
%K 钾通道
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=3E3CAC4DDDAF2F71CB7E7F120E8217A3&yid=F53A2717BDB04D52&vid=5D311CA918CA9A03&iid=E158A972A605785F&sid=D7F3FD6D87AEF622&eid=245EE63BFE8F8B66&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=1