%0 Journal Article
%T Heterologous Genes Expression on Escherichia coli Chromosome lac Operon Using Red Recombination<br>利用Red重组工程技术解决外源基因在大肠杆菌染色体lac操纵子中的表达
%A Shanhu Li
%A Qingguo Shi
%A Cuifen Huang
%A Jianguang Zhou
%A <br>李山虎
%A 施庆国
%A 黄翠芬
%A 周建光
%J 生物工程学报
%D 2008
%I 
%X To achieve efficient and stable expression of heterologous exogenetic protein or antigen in E. coli chromosome, the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology. The quantitative analysis of exogenous gene expression indicated that the target gene could be efficiently expressed at lacZ site of lac operon. The results confirmed the efficient screening and stable expression of heterologous protein or antigen on chromosome by using the recombinant engineering technique. This study demonstrated that the chromosome could be used as a vector for heterologous protein or antigen and the stable expression of exogenous gene on E. coli chromosome had no side effect on the bacterial growth and propagation.
%K recombination
%K homologous recombination
%K luciferase report gene
%K prokaryotic expression<br>重组工程
%K 同源重组
%K 荧光素酶报告基因
%K 原核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F5C69DF060F789265568BCB06E1E35A0&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=39E48869A719B9DE&eid=28B3EB92D5061EA4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=12