%0 Journal Article
%T Development and application of TaqMan-MGB real-time quantitative PCR assay for detection of goat pox virus<br>山羊痘病毒TaqMan-MGB荧光定量PCR检测方法的建立与应用
%A Zhentao Cheng
%A Jun Yue
%A Yongming Li
%A Leren Xu
%A Kaigong Wang
%A Bijun Zhou
%A Junyi Chen
%A Jun Li
%A Nan Jiang
%A <br>程振涛
%A 岳筠
%A 李永明
%A 许乐仁
%A 王开功
%A 周碧君
%A 陈军义
%A 李俊
%A 江楠
%J 生物工程学报
%D 2009
%I 
%X The complete gene sequences of eight capripoxvirus strains in GenBank were aligned and analyzed with DNAStar software. We selected a size of 64 bp gene fragment that was located in gp064 region of goat pox virus (GPV) genome, and designed a pair of primers and a TaqMan-MGB probe against the gene fragment with Primer Express 2.0 software. Then, the fluorescence quantitative PCR (FQ-PCR) assay was developed and the standard curve of different dilution series was described. We extracted the DNA samples from cl...
%K goat pox virus
%K TaqMan-MGB probe
%K fluorescence quantitative PCR
%K development
%K application<br>山羊痘病毒
%K TaqMan-MGB探针
%K 荧光定量PCR
%K 建立
%K 应用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8DBF2DC15DA786BF1FA2337A743248F6&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=38B194292C032A66&sid=1A033C02510EFBE6&eid=7D1E6EEC2019967D&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14