%0 Journal Article
%T Kinetic mechanisms of glycerol dehydrogenase and 1,3-propanediol oxidoreductase from Klebsiella pneumoniae<br>克雷伯杆菌甘油脱氢酶和1,3-丙二醇氧化还原酶的动力学机制
%A Hongwen Chen
%A Jinfeng Nie
%A Guo Chen
%A Baishan Fang
%A <br>陈宏文
%A 聂金峰
%A 陈国
%A 方柏山
%J 生物工程学报
%D 2010
%I 
%X The kinetic mechanisms of two key enzymes in the biotransformation of glycerol to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae, glycerol dehydrogenase (GDH) and 1,3-propanediol oxidoreductase (PDOR), was characterized. Kinetics on initial velocity and product inhibition revealed that GDH and PDOR follow an ordered Bi-Bi sequential mechanism. Kinetic models for GDH and PDOR showed that the oxidation reaction catalyzed by GDH was the rate-limiting step in coupled enzymatic reaction when the GDH/PDOR was 1:1, and the NAD+ was the main form of coenzyme in the reaction. Knowledge about the kinetic mechanisms will be helpful to understand how these enzymes is regulated, which will be useful for further enzyme catalysis and metabolic engineering studies.
%K glycerol dehydrogenase
%K 1
%K 3-propanediol oxidoreductase
%K ordered Bi-Bi mechanism
%K kinetic model<br>甘油脱氢酶，1
%K 3-丙二醇氧化还原酶，有序BiBi机制，动力学模型
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=09E06DA7BB67E829F7625CFC3F281E89&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=6425DAE0271BB751&eid=B1F98368A47B8888&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0