%0 Journal Article
%T Cloning of Keratinocyte growth factor 2 gene (KGF2) and its transformation to Brassica napus L.<br>角质细胞生长因子2基因 (KGF2) 的克隆及其对油菜的遗传转化
%A Guoqing Pan
%A Shuang Zhang
%A Xiuming Liu
%A Ying Li
%A Yaofang Zhang
%A Hongzhi Li
%A Haiyan Li
%A Xiaokun Li
%A <br>潘国庆
%A 张爽
%A 刘秀明
%A 李营
%A 张耀方
%A 李洪志
%A 李海燕
%A 李校堃
%J 生物工程学报
%D 2010
%I 
%X Recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. In the present study, the cDNA of the human gene keratinocyte growth factor 2 (KGF2) was replaced with plant preferred codons by PCR, and the modified full-length cDNA was cloned into the plant expression vector pCAMBIA-YO containing the oil-body promoter. The fusion construct pCAMBIA-YO-KGF2 was transformed into Brassica napus by Agrobacterium tumefacien-mediated cotyledon transformation method. The transgenic seedlings were identified by PCR, Southern and western blot analysis all showed that KGF2 gene was successfully expressed in in transgenicBrassica napus.
%K Brassica napus L
%K transgenic plants
%K plant bioreactor
%K keratinocyte growth factor<br>油菜，转基因植株，植物生物反应器，角质细胞生长因子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=DDAFB12C634E9689C4F0335CF81EAFD4&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=B31275AF3241DB2D&sid=70081086C74F4110&eid=E3691231514F8E11&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0