%0 Journal Article
%T Metabolic flux analysis of L-serine synthesis by Corynebacterium glutamicum SYPS-062<br>谷氨酸棒杆菌SYPS-062合成L-丝氨酸的代谢通量分析
%A Xiaomei Zhang
%A Wenfang Dou
%A Hongyu Xu
%A Zhenghong Xu
%A <br>张晓梅
%A 窦文芳
%A 许泓瑜
%A 许正宏
%J 生物工程学报
%D 2010
%I 
%X Corynebacterium glutamicum SYPS-062 was an L-serine producing strain stored at our lab and could produce L-serine directly from sugar. We studied, the effects of cofactors in one carbon unit metabolism-folate and VB12 on the cell growth, sucrose consumption and L-serine production by SYPS-062. In the same time, the metabolic flux distribution was determined and different conditions. The supplementation of folate or VB12 enhanced the cell growth, energy synthesis, and finally increased the flux of pentose phosphate pathway (HMP), whereas the carbon flux to L-serine was decreased. The addition of VB12 not only increased the ratio of L-serine synthesis pathway on G3P joint, but also caused the insufficiency of tricarboxylic acid cycle (TCA ) flux, which needed more anaplerotic reaction flux to replenish TCA cycle, that was an important limiting factor for the further increasing of the L-serine productivity.
%K Corynebacterium glutamicum
%K L-serine
%K one carbon units
%K metabolic flux analysis<br>谷氨酸棒杆菌，L-丝氨酸，一碳单元，代谢通量分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=57E8BEBBD1DDABACD37F2F7DD22498FE&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=F3090AE9B60B7ED1&sid=196264D95F295743&eid=40DE18199B7CA9BB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0