%0 Journal Article
%T A Modified TAIL-PCR and Its Application in Isolating Gene Promoter of Wheat<br>TAIL-PCR的改良及其在分离小麦基因启动子中的应用
%A Yanguang Qiu
%A Jinghan Tian
%A Rongchao Ge
%A Baocun Zhao
%A Yinzhu Shen
%A Zhanjing Huang
%A <br>仇艳光
%A 田景汉
%A 葛荣朝
%A 赵宝存
%A 沈银柱
%A 黄占景
%J 生物工程学报
%D 2008
%I 
%X Using a modified TAIL-PCR technique, the 5' -flanking region of the X gene in wheat was successfully isolated. Two novel modifications of the TAIL-PCR were introduced here: using a battery of random 10-mers as the short arbitrary primers instead of three degenerate 16-mers; using 29 degrees C instead of 44 degrees C as the annealing temperature for the low-stringency cycle; increasing five high-stringency cycles and reducing five low-stringency cycles; and using single primers for the third round of product identification. Isolated 5' -flanking region was fused to the GUS gene, and tested for expression in Arabidopsis plants. Histochemical analysis of the transgenic plants showed the report gene was driven by isolated 5'-flanking region. Modified TAIL-PCR technique could isolate rapidly the promoter of any gene from organisms with large genomes.
%K TAIL-PCR
%K RAPD (random amplified polymorphic DNA)
%K promoter<br>热不对称交错PCR
%K 随机引物
%K 启动子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=ABB7EE4FC31F150B56173E7D97FFAAE2&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=08B2E838F29A693A&eid=C230D48775108CE3&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=5