%0 Journal Article
%T Ovine Follistatin gene expression and functional analysis of follistatin domains<br>绵羊Follistatin基因表达及其结构域的功能分析
%A Ning Zhang
%A Xuemei Zhang
%A Mingjun Liu
%A Lixin Tan
%A <br>张宁
%A 张雪梅
%A 刘明军
%A 谭立新
%J 生物工程学报
%D 2010
%I 
%X In order to study ovine follistatin function, we amplified the total of 1 038 base pair of ovine complete follistatin cDNA and cloned into pGEM-T vector by RT-PCR from ovine ovary RNA. After removal of the signal peptide it was subcloned into the pET41a to construct the prokaryotic expression vector, named pFSsig?. SDS-PAGE and Western blotting identified the 66 kDa product of the expression of follistatin cDNA. Based on the complete CDS sequence, we cloned follistatin N-terminal domain and domain 1 with PCR and inserted into pLEX-MCS lentiviral vector, named pFS-N+D1. After package and passage of lentivirus in 293T cells, and then infected sheep primary muscle cells (SPMC). The expression of FS N+D1 in SPMC was assayed by Western blotting. The cell growth curve of the infected SPMC and noninfected control cells displayed that FS N+D1 stablly transfected SPMC proliferated significantly faster than the control cells (P<0.01). Our data inferred that ovine FS N+D1 domain had the function to stimulate sheep muscle cell growth.
%K ovine Follistatin gene
%K prokaryotes expression
%K lentivirus
%K cell growth curve<br>绵羊Follistatin基因，原核表达，慢病毒，细胞生长曲线
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=23EBCAF66AA51913955C0A5C24AD63F7&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=5D311CA918CA9A03&sid=208AC7DC28BD1EC6&eid=0C7160AA184DEC96&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0