%0 Journal Article
%T Optimization of Bacterial Cellulose Fermentation Medium and Observation of Bacterial Cellulose Ultra-micro-structure<br>细菌纤维素发酵培养基的优化及超微观结构分析
%A Ruiqin Wu
%A Shuangkui Du
%A Zhixi Li
%A Xiaohui Xing
%A Dongyan Shao
%A Yanli Fan
%A Bo Li
%A Xueliang Zhang
%A Lijun Bu
%A <br>毋锐琴
%A 杜双奎
%A 李志西
%A 邢晓晖
%A 邵东燕
%A 樊艳丽
%A 李博
%A 张学良
%A 布丽君
%J 生物工程学报
%D 2008
%I 
%X In order to improve the yield of bacterial cellulose (BC), the fermentation medium of BC-producing strain J2 (Gluconobacter) was optimized, and BC ultra-micro-structure was observed. Initially, Plackett-Burman design was employed to evaluate eight variables which were relevant to BC production. Three statistically significant parameters including yeast extract, ZnSO4, ethanol were selected and other 5 variables were not significant (P>0.05). The optimized levels of three variables were defined by Box-Behnken design and response surface methodology (RSM). BC ultra-micro-structure was observed by scanning electron microscope (SEM) with cotton cellulose as comparison. The results indicated that the BC yield under the optimum fermentation medium was 11.52 g/100 mL, which was as 1.35 times as that under the original fermentation medium. The SEM photos manifested that bacterial cellulose ribbon, with a diameter less than 0.1 mm, was less than cotton cellulose ribbon. The bacteria inside the cellulose net were eliminated after the NaOH treatment.
%K Plackett-Burman design
%K Box-Behnken design
%K response surface methodology
%K optimization of fermentation medium
%K ultra-micro-structure<br>Plackett-Burman设计
%K Box-Behnken设计
%K 响应面分析法
%K 培养基优化
%K 超微观结构
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FE8EEA9888EB85D634822F3CCE762BF3&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=B31275AF3241DB2D&sid=305A58D956DD6BAF&eid=CD794621A184DC14&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=13