%0 Journal Article
%T Construction and Immunogenicity Analysis of Antigenic Epitopes of Swine Influenza Virus<br>猪流感病毒抗原表位基因表达载体构建与免疫原性分析
%A Huili Liu
%A Jilan Xing
%A Jie Pan
%A Qiufeng Yang
%A Yanmin Zhao
%A <br>刘惠莉
%A 邢继兰
%A 潘洁
%A 杨秋峰
%A 赵艳敏
%J 生物工程学报
%D 2008
%I 
%X Several antigen epitopes were designed according to the sequences of Swine influenza virus hemagglutinin (HA) genes and lined with the interval. The gene was amplified by PCR and sub cloned into pET30a (+) vector. The fusion protein was expressed in E. coli BL21 (DE3) by induced with IPTG and purified by affinity chromatography. The molecular weight of the protein was about 20 kD in SDS-PAGE. Immunological activity of the fusion protein was analyzed by Western blot. The results showed that the fusion protein could interact with anti-His antibody and the rabbit antiserum against SIV. The immunized mouse can produced antibodies against the target peptide and HI antibody against SIV H1N1 or H3N2. This study provides a new vaccine candidate for SIV.
%K swine influenza virus
%K antigen epitopes
%K new vaccine<br>猪流感病毒
%K 抗原表位
%K 新型疫苗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=1337963D816E50349A3DEFAE6B4C9E66&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=DC2727B6AC017B3F&eid=D02611D1F8166C9A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15