%0 Journal Article
%T Ganoderma lucidum immunomodulatory protein(Lz-8) expressed in Pichia pastoris and the identification of immunocompetence<br>灵芝免疫调节蛋白(Lz-8)在毕赤酵母中的表达及其免疫活性鉴定
%A Chongyang Liang
%A Shuqin Zhang
%A Zhiyi Liu
%A Fei Sun
%A <br>梁重阳
%A 张淑芹
%A 刘志屹
%A 孙非
%J 生物工程学报
%D 2009
%I 
%X Fungi immunoregulatory proteins family is effective in immunological regulation and anti-tumor. We used Pichia pastoris expression system for recombinant expression of Lz-8, an immunomodulatory protein isolated from fruiting body of Ganoderma lucidum. The Gs115 (mut+) strains of P. pastoris was used as host cells. PCR and sequencing of DNA showed that Lz-8 cDNA was successfully integrated into the P. pastoris genome. Electrophoresis(SDS-PAGE), matrix assisted laser desorption ionisation time-of-flight mass ...
%K Ganoderma lucidum
%K immunomodulatory protein
%K Pichia pastoris
%K immunolomodulation<br>灵芝
%K 免疫调节蛋白
%K 毕赤酵母
%K 免疫蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A1DD61A3349A40AD922AF714009797D3&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=38B194292C032A66&sid=5319469C819FCFF1&eid=C66DE7562B0326E2&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=16