%0 Journal Article
%T Development and application of perfusion culture producing seed cells in WAVETM bioreactor<br>WAVETM生物反应器灌注培养生产种子细胞的开发和应用
%A Jianjun Yang
%A Lili Sui
%A <br>杨建军
%A 隋礼丽
%J 生物工程学报
%D 2012
%I 
%X In recent years, Chinese hamster ovary (CHO) production vessel volume has reached more than 1 000 L in Chinese biopharms, and 10 000 L in foreign big biopharms, such as Lonza and Genetech. In general, there are some steps seed bioreactor for seed expansion, which decreases the efficiency of production process. In this work, a perfusion-based process was developed to drastically increase the split ratio during the scale-up of CHO cell cultures. Fed-batch cultures were inoculated with cells propagated in either batch or perfusion cultures that grown in disposable Cellbags using the WAVETM Bioreactor system. The higher cell concentration of 2×107 cells/mL with 95% viability allowed to increase the split ratio to about 1:50~1:100 for inoculum propagated in perfusion culture. The method described here could reduce the number of required expansion steps and eliminate two or three bioreactors. Disposable perfusion bioreactor with only a few liters working volume have the potential to directly inoculate volumes of up to 1 000 liters. This would allow to shorten process time in these bioreactors, which often are the bottleneck in plant throughput.
%K WAVETM Bioreactor
%K perfusion culture
%K seed-train<br>WAVETM波浪式生物反应器
%K 灌注培养
%K 种子罐
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A9B497033F261F941AB4076BFFDB6EC2&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=38B194292C032A66&sid=EF78DD85C21CB57F&eid=4C2B9916B58305BE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0