%0 Journal Article
%T Establishing the regeneration system of sunflower<br>向日葵离体再生体系的建立
%A Yuanyuan Wang
%A Caifeng Li
%A Yifei Zhang
%A Yeting Chen
%A Liying Zhao
%A Peng Yue
%A Xiangyong Teng
%A Nanbo WANG
%A <br>王园园
%A 李彩凤
%A 张翼飞
%A 陈业婷
%A 赵丽影
%A 越鹏
%A 滕祥勇
%A 王南博
%J 生物工程学报
%D 2011
%I 
%X In order to establish a high efficient regeneration system of sunflower, we optimized the process of callus induction, differentiation and rooting by screening the optimum genotype, explant materials, hormone and cytokine concentration and additives. The results indicated that hybrid sunflowers were easier to regenerate than selfing ones; The best explant was four days cotyledon. The optimum induction medium was Murashige and Skoog (MS) +2.0 mg/L 6-benzyladenine (6-BA)+0.5 mg/L naphthaleneacetic acid (NAA)+1.0 mg/L kinetin (KT). The maximum rate of callus induction was 100%. The optimum differentiation medium was MS +0.2 mg/L 6-BA +0.5 mg/L NAA +0.3 mg/L KT +0.3 mg/L silver nitrate (AgNO3)+0.2 g/L active carbon (AC), and the buds differentiation rate was up to 71%. The best rooting culture medium was 1/2 MS +0.6 mg/L indolebutyric acid (IBA). The highest rooting rate was 77%. The analysis of variance showed that genotype, explants growth time, different kinds and concentration of hormone, AC concentration had a significant effect on sunflower regeneration.
%K sunflower
%K vitro regeneration
%K callus induction
%K bud differentiation
%K rooting<br>向日葵，离体再生，愈伤组织诱导，芽分化，生根
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F437B1BF01E7F486C340FC66BF47D524&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=9CF7A0430CBB2DFD&sid=F2D4C4F6570F167A&eid=79D108842269596A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=28