%0 Journal Article
%T Screening of Clostridium strains through ribosome engineering for improved butanol production<br>利用核糖体工程选育丙酮丁醇菌提高丁醇产量
%A Lijie Chen
%A Guanglai Shang
%A Wenjie Yuan
%A Youduo Wu
%A Fengwu Bai
%A <br>陈丽杰
%A 商光来
%A 袁文杰
%A 吴又多
%A 白凤武
%J 生物工程学报
%D 2012
%I 
%X We used ribosome engineering technology, with which antibiotic-resistant strains are resulted from mutations on microbial ribosome, to screen a high butanol-producing Clostridium strain. A novel mutant strain S3 with high butanol production and tolerance was obtained from the original Clostridium acetobutylicum L7 with the presence of mutagen of streptomycin. Butanol of 12.48 g/L and ethanol of 1.70 g/L were achieved in S3, 11.2% and 50%, respectively higher than the parent strain. The conversion rate of glucose to butanol increased from 0.19 to 0.22, and fermentation time was 9 h shorter. This caused an increase in butanol productivity by 30.5%, reaching 0.24 g/(L?h). The mutant butanol tolerance was increased from 12 g/L to 14 g/L, the viscosity of fermentation broth was dramatically decreased to 4 mPa/s, 60% lower than the parent strain. In addition, the genetic stability of mutant strain S3 was also favorable. These results demonstrate that ribosome engineering technology may be a promising process for developing high butanol-producing strains.
%K ribosome engineering
%K Clostridium acetobutylicum
%K butanol fermentation
%K butanol tolerance
%K streptomycin<br>核糖体工程
%K 丙酮丁醇梭菌
%K 丁醇发酵
%K 丁醇耐性
%K 链霉素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=22653CDAF59BBDC135139F3CF9980ACB&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=9CF7A0430CBB2DFD&sid=94D812A784CFA7CC&eid=10343777FBBF55D5&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0