%0 Journal Article
%T Effects of calcium on synthesis of rosmarinic acid and related enzymes in suspension cultures of Salvia miltiorrhiza<br>Ca2+对丹参培养细胞中迷迭香酸合成及其相关酶活性的影响
%A 刘连成
%A 董娟娥
%A 张婧一
%A 党小琳
%A 行冰玉
%A 杨喜玲
%J 生物工程学报
%D 2012
%I 
%X We studied the influence of the concentration of Ca2+ (0~50 mmol/L) in culture medium on the synthesis of rosmarinic acid (RA) and related enzymes in Salvia miltiorrhiza suspension cultures. Using verpamil (VP, a calcium channel antagonist) and ionophore A23187, we studied the mechanism of secondary metabolites of Salvia miltiorrhiza suspension cultures influenced by the concentration of Ca2+ in the culture medium. The synthesis of intracellular RA in 6-day incubation was significantly dependent on the medium Ca2+ concentration. At the optimal Ca2+ concentration of 10?mmol/L, a maximal RA content of 20.149 mg/g biomass dry weight was reached, which was about 37.3% and 20.4% higher than that at Ca2+concentrations of 1 and 3 mmol/L, respectively. The variation of the activity of PAL and TAT, two key enzymes of the two branches of RA, could be affected by the concentration of Ca2+ in culture medium. The change of their activity occurred prior to the accumulation of RA, which suggested both of the key enzymes be involved in the synthesis of RA. Meanwhile, the enzymatic action of PAL was more distinct than TAT. The treatment of VP and A23187, respectively, indicated that the influence of RA affected by the concentration of Ca2+ in the culture medium was accomplished by the intracellular Ca2+, and the flow of Ca2+ from the extracellular to the intracellular environment could also participate in this process.
%K Salvia miltiorrhiza
%K rosmarinic acid
%K calcium ion
%K phenylalanine ammonialyase
%K tyrosine aminotransferase<br>丹参，迷迭香酸，钙离子，苯丙氨酸解氨酶，酪氨酸氨基转移酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=324F8504B27098567FE2F464909B49C0&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=708DD6B15D2464E8&sid=D98C4F25072149E5&eid=01471B003B2963CC&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0