%0 Journal Article
%T Expression and bioactivity effects to Hela of recombinant toxin protein rLj-RGD3 from Lampetra japonica<br>重组七鳃鳗细胞毒素蛋白rLj-RGD3表达及对Hela细胞活性的影响
%A Piqiao Zhang
%A Jihong Wang
%A Xin Liu
%A Dan Chu
%A Qingwei Li
%A <br>张丕桥
%A 王继红
%A 刘欣
%A 褚丹
%A 李庆伟
%J 生物工程学报
%D 2009
%I 
%X Lj-RGD3 was a toxin from the saliva gland of Lampetra japonica.To study the anti-tumor function of rLj-RGD3 and confirm its biological status and significance, we extracted total RNA from the saliva gland and amplified the cDNA of Lj-RGD3 by RT-PCR.The cDNA of Lj-RGD3 was 357 bp long and encoded a polypeptide composed of 118 amino acids including 2 cysteines, 17 histidines and 3 RGD(Arg-Gly-Asp) motifs.We cloned the cDNA into the plasmid pET23b, and expressed the recombinant protein rLj-RGD3 in Escherichia ...
%K Lampetra japonica
%K RGD-motif
%K integrin
%K gene recombinant
%K anti-tumor<br>七鳃鳗
%K RGD模体
%K 整合素
%K 基因重组
%K 抗肿瘤
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D19A99076E5613C5A49422D6C3888BE2&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=94C357A881DFC066&sid=710C005323C0774A&eid=D02611D1F8166C9A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=29