%0 Journal Article
%T Expression and Activity Analysis of Catalytic Domain of PTP1B<br>蛋白酪氨酸磷酸酶PTP1B催化活性区的原核表达及活性分析
%A Shengyu Wang
%A Jianghua Yan
%A Yanglin Pan
%A Xuejun Li
%A Zhong Chen
%A <br>王生育
%A 颜江华
%A 潘阳霖
%A 李学军
%A 陈忠
%J 生物工程学报
%D 2008
%I 
%X The amino acid sequence (1-301aa) coding the human PTP1B catalytic domain (PTP1Bc) was obtained from the GenBank. The PTP1Bc gene was constructed by overlapping PCR, then was inserted into vector pET-22b(+) and expressed efficiently in E. coli BL21(DE3) under optimum condition after IPTG induction. The proteins were expressed mainly as inclusion bodies with the yield of more than 30% of total bacterial proteins. The expressed products were purified through Ni2+-affinity chromatographic column. After purification, the purity of the proteins was more than 95%. Western blotting analysis suggested that the purified proteins could specially combine with anti-PTP1B antibody. Enzyme activity assay showed that the protein has phosphatase activities.
%K PTP1Bc
%K expression
%K protein purification
%K activity analysis<br>PTP1Bc
%K 基因表达
%K 蛋白纯化
%K 活性分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=59D3F6218CDB15E994001E613C3322C4&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=4AB4178709047BE3&eid=2E15A588990CC690&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=13