%0 Journal Article
%T Optimization of Method for Detecting the Activation of Potassium Channels in Rat Cardiac Muscle Cells<br>检测心肌细胞钾离子通道蛋白活化水平方法的优化
%A Xi Wang
%A Lei Zhang
%A Shisheng Zhou
%A Wei Zou
%A <br>王曦
%A 张磊
%A 周士胜
%A 邹伟
%J 生物工程学报
%D 2008
%I 
%X To detect the phosphorylation of potassium channel protein (Kv1.2 and Kv1.5) in rat cardiac muscle cells accurately, we applied the combined method of immunoprecipitation and Western blot in this study. Compared with using Western blot alone, the combination of immunoprecipitation and Western blot displayed high sensitivity to detect the activation of potassium channel proteins. Because of its simplicity, quickness and reproducibility, we find that this method was promising for detecting the phosphorylation of Kv1.2 and Kv1.5 proteins or other potassium channel proteins in rat cardiac muscle cells.
%K immunoprecipitation
%K rats cardiac muscle cells
%K potassium channel proteins<br>蛋白质免疫沉淀
%K 大鼠心肌细胞
%K 钾离子通道蛋白
%K 检测方法
%K 大鼠心肌细胞
%K 离子通道蛋白
%K 活化
%K 表达水平
%K 优化
%K Cells
%K Cardiac
%K Muscle
%K Potassium
%K Channels
%K Activation
%K Detecting
%K Method
%K 敏感性
%K 实验方案
%K 蛋白磷酸化
%K 钾通道
%K 亚家族
%K 白及
%K 钾离子通道
%K 比较
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4D2F4FA8B7CC4FE74622F000961208AD&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=68FF5306A8F9C315&eid=500A64A3035F6B9C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=11