%0 Journal Article
%T Purification of anti-HBcAg monoclonal antibodies using immobilized metal ion affinity chromatography<br>金属螯合亲和层析法纯化抗乙肝核心抗原单克隆抗体
%A Ji Zhu
%A Yu Yi
%A Yinfei Wu
%A Keyin Zhu
%A Jianfeng Mei
%A Jianshu Chen
%A Guoqing Ying
%A <br>祝骥
%A 易喻
%A 吴银飞
%A 朱克寅
%A 梅建凤
%A 陈建澍
%A 应国清
%J 生物工程学报
%D 2009
%I 
%X Anti-HBcAg monoclonal antibodies from mouse ascites were purified by using immobilized metal ion affinity chromatography. We optimized the conditions of sample loading and elution. The results showed that when the pH stepwise elution was used, the best solution for sample loading was 20 mmol/L phosphate buffer containing 0.5 mol/L sodium chloride at pH 8.0 and the mAb was eluted at pH 5.0. The purity of obtained mAb was more than 85% and recovery reached 80%. When the adsorbed proteins were eluted by using ...
%K immobilized metal ion affinity chromatography
%K monoclonal antibody
%K Hepatitis B core antigen
%K mouse ascites<br>金属螯合亲和层析
%K 单克隆抗体
%K 乙肝核心抗原
%K 小鼠腹水
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0FB33A60EB6629085CBF6CC98C3BDF8A&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=F3090AE9B60B7ED1&sid=FAE014814EB8ABBA&eid=6DC9B9BB39B3E540&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0