%0 Journal Article
%T Endothelial genesis inhibitor-8t(EDI-8t)against tumor growth<br>抑制肿瘤生长活性的内皮生长抑制剂EDI-8t
%A Qingwei Zhou
%A Peng Du
%A Yue Qian
%A Qian Zhang
%A Baoshan Feng
%A Hongzhen Ding
%A Renbao Gan
%A Hui Zhang
%A <br>周庆玮
%A 杜鹏
%A 钱悦
%A 张倩
%A 冯宝山
%A 丁红珍
%A 甘人宝
%A 张辉
%J 生物工程学报
%D 2010
%I 
%X On the basis of the origin comparison of known endothelial genesis inhibitors, a 417-bp cDNA fragment was amplified from umbilical cord by RT-PCR and cloned into the expression vector pPIC9, followed by transformation into Pichia pastoris GS115. The resulted yeast was induced with methanol to express recombinant protein. The resulted protein was purified from culture broth and designated as EDI-8t. The in vitro study showed that EDI-8t, originated from collagen VIII, could specifically inhibit the growth and migration of bovine aortic endothelial cells (BAEC) stimulated by basic fibroblast growth factor (bFGF). The protein also exhibited the activity to cause cell apoptosis. In vivo EDI-8t showed the identical activity comparing with endostatin to inhibit the growth of liver tumor transplanted into nude mice. Interestingly, EDI-8t showed higher activity than endostatin to inhibit tumor growth in metastatic model of melanoma mice.
%K endothelial genesis inhibitor
%K EDI-8t
%K tumor<br>内皮细胞生长抑制剂，EDI-8t，肿瘤
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0ED9B3F63E29A89EAB2A350327D84BA0&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=59906B3B2830C2C5&sid=A84A3AD26CE1D4D2&eid=48603EE1050A0BB7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0