%0 Journal Article
%T Applications and perspectives of DNA stable-isotope probing in metagenomics: a review<br>DNA稳定同位素探针技术在宏基因组学中的应用及前景
%A Wei Liu
%A Xiao Wei
%A Jing Yuan
%A Liuyu Huang
%A <br>刘威
%A 魏晓
%A 袁静
%A 黄留玉
%J 生物工程学报
%D 2011
%I 
%X DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds. We summarize recent progress in SIP-metagenomic techniques and applications and discuss prospects for this combined approach in environmental microbiology and biotechnology.
%K metagenomics
%K stable-isotope probing
%K microecosystem
%K DNA-SIP<br>宏基因组学，同位素探针，微生态，DNA-SIP
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=95B9D868E90825F260FFB1E237A262E4&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=92DA076AF6760FAC&eid=6313C162FF75889A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=44