%0 Journal Article
%T Influence of Japanese enciphalitis virus capsid protein on the self-replicate ability of JEV replicon vectors<br>日本脑炎病毒C蛋白对其复制子载体自主复制活性的影响
%A Ying Huang
%A Shan Liu
%A Peng Yang
%A Chao Wang
%A Yun Du
%A Zhiwei Sun
%A Weiyuan Yu
%A <br>黄莺
%A 刘珊
%A 杨鹏
%A 王超
%A 杜韫
%A 孙志伟
%A 俞炜源
%J 生物工程学报
%D 2010
%I 
%X To optimize a self-replicate Japanese enciphalitis virus (JEV) replicon, and to make it as an efficient vector to express the heterologous protein, we constructed three JEV replicons by PCR-based shortening the length of capsid genes. The vectors remained full or part of C gene, based on the JEV replicon pCTCJEV. Lac Z was selected as the reporter gene to verify the self-replicate ability of these DNA-based replicons. While transfected into the cell lines CME-4, which continuously expressing the JEV structure proteins C-prM-E, the JEV replicons pCMW-2M-1LACZ, pCMW-2M-3LACZ, which remained the first 23aa and 68aa of C protein, can express the reporter protein as the same level as pCMW-2M-LACZ with the full-length C protein. These results illustrated that the JEV replicon vector with 69-nt of the C gene can retain the self-replicate ability, and provide valuable tools to construct a possible vector for a long-lasting JEV RNA virus expression system.
%K Japanese enciphalitis virus (JEV)
%K C protein
%K replicon vector<br>JEV，C蛋白，复制子载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=293C9B171D0ADDAFC67D3B306BCD09FF&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=5D311CA918CA9A03&sid=61EDB4BBA42E40FF&eid=6B48C3DA6EA0194C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0