%0 Journal Article
%T Transformation of enhanced green fluorescent protein gene in Paecilomyces lilacinus mediated by Agrobacterium tumefaciens<br>根癌农杆菌介导的增强型绿色荧光蛋白基因在淡紫拟青霉中的转化
%A Jieping Wang
%A Jiaxu Wang
%A Fan Liu
%A Cangsang Pan
%A <br>王阶平
%A 汪家旭
%A 刘凡
%A 潘沧桑
%J 生物工程学报
%D 2010
%I 
%X The main aim of this study was to transform the enhanced green fluorescent protein gene (egfp) into biocontrol fungus Paecilomyces lilacinus strain 9410. We constructed the expression vector pUPNGT of the fusion gene nptII-egfp using pcDNA3.1(-) as a helper plasmid. The egfp gene was then transformed into P. lilacinus strain 9410 via Agrobacterium tumefaciens-mediated transformation. PCR and Southern blotting analysis showed that the egfp gene was integrated into the genomes of the tested transformants and the integration manner was single-copy. The transformants could generate green fluorescence when they were excited by 488 nm blue laser. These results indicated that the egfp gene had been successfully transformed into P. lilacinus 9410 and expressed in the tested transformants. Our work may provide a new approach to assess environmental safety and practical biocontrol efficacy of P. lilacinus under different conditions.
%K Paecilomyces lilacinus
%K enhanced green fluorescent protein
%K Agrobacterium tumefaciens
%K genetic transformation<br>淡紫拟青霉，增强型绿色荧光蛋白，根癌农杆菌，遗传转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2819623A6B76AF6154A03DCFE5B97BBE&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=94C357A881DFC066&sid=039DCCB9394D9766&eid=20ED669EB429E15C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0