%0 Journal Article
%T Expression of a protein elicitor pebC1 from Botrytis cinerea in Pichia pastoris<br>灰葡萄孢菌激发子pebC1在毕赤酵母中的分泌表达及其生物活性分析
%A Zhang Yunhua
%A Yang Xiufen
%A Liu Yanfeng
%A Yu Shanjiang
%A Qiu Dewen
%A <br>Zhang Y
%A Yang X
%A Liu Y
%A Yu S
%A Qiu D
%J 生物工程学报
%D 2011
%I 
%X In order to express pebC1 in Pichia pastoris, the pebC1 sequence was amplified from genome Botrytis cinerea BC-4-2-2-1 by PCR and subcloned into the Pichua pastoris expression vector pPIC9K to generate pPIC9K-pebC1. The recombinant plasmid was linearized by Bgl II and transformed into Pichia pastoris GS115 by electroporation. Recombinant Pichia pastoris GS115/pPIC9K-pebC1 was screened by MD and G418-YPD plates and further confirmed by PCR. The protein expression was induced by methanol and analyzed by SDS-PAGE. SDS-PAGE analysis showed a special band about 39 kDa and western blotting indicated a good antigenicity of the expressed protein. Bioassay results showed that the recombinant protein PebC1 can induce resistance to gray mould disease of cucumber and Arabidopsi thaliana.
%K elicitor
%K pebC1 gene
%K Pichia pastoris
%K biological activity<br>激发子，pebC1基因，毕赤酵母，生物活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F437B1BF01E7F48620A5716806CCC58D&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=708DD6B15D2464E8&sid=95B0C98E7814901D&eid=FDDE90F0514924E3&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=21