%0 Journal Article
%T Enzymes related with NAD synthesis promote conversion of 1,4-butanediol to 4-hydroxybutyrate<br>烟酰胺腺嘌呤二核苷酸合成相关酶有助于1,4-丁二醇转化为4-羟基丁酸
%A Xin Zhang
%A Guoqiang Chen
%A <br>张鑫
%A 陈国强
%J 生物工程学报
%D 2011
%I 
%X Besides medical application, 4-hydroxybutyrate (4-HB) is a precursor of P3HB4HB, a bioplastic showing excellent physical properties and degradability. Escherichia coli S17-1 (pZL-dhaT-aldD) can transform 1, 4-butanediol (1,4-BD) into 4HB with participation of cofactor NAD. To enhance productivity, nicotinic acid phosphoribosyltransferase (PncB) and nicotinamide adenine dinucleotide synthetase (NadE) were overexpressed to increase intracellular nicotinamide adenine dinucleotide concentration and promote reaction process. The shake flask fermentation result showed that the conversion rate increased by 13.03% with help of PncB-NadE, leading to 4.87 g/L 4HB from 10 g/L 1,4-BD, and productivity was increased by 40.91% to1.86 g/g. These results demonstrated that expression of PncB and NadE is beneficial for conversion of 1,4-BD to 4HB.
%K 4-hydroxybutyric acid
%K 1
%K 4-butanediol
%K nicotinamide adenine dinucleotide (NAD)
%K nicotinic acid phosphoribosyltransferase
%K NAD synthetase<br>4-羟基丁酸，1
%K 4-丁二醇，烟酰胺腺嘌呤二核苷酸
%K (NAD)，烟酸磷酸核糖转移酶，烟酰胺腺嘌呤二核苷酸合成酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4D541C05C7575AEA622626DDA89093A5&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=59906B3B2830C2C5&sid=F8ACF30D915F587C&eid=7820732DED23DCED&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=27