%0 Journal Article
%T Heterologous expression of stearoyl-CoA desaturase-1 in Lactococcus lactis NZ3900<br>硬脂酰-辅酶A脱氢酶基因在食品级乳酸菌中的表达
%A Lamei Wang
%A Shili Li
%A Kemian Gou
%A Yuzhu Luo
%A <br>王腊梅
%A 李世丽
%A 苟克勉
%A 罗玉柱
%J 生物工程学报
%D 2012
%I 
%X The possibility of heterologous expression of human Stearoyl-CoA Desaturase(scd1) was investigated.The scd1 encoding sequence was inserted into the pNZ8149 to generate the pNZ8149-scd1 expression plasmids.Then we introduced the pNZ8149-scd1 construct into the Lactococcus lactis NZ3900 to investigate its enzyme activity.The results show that heterologous expressed SCD1 enzyme resulted in a 92%–169% increase in the C16:1n-7 and a 53-127% increase in the C18:1n-7(P<0.05).The SCD1 enzyme was capable of producing n-7 fatty acids in Lactococcus lactis efficiently.It also suggests that the fatty acid desaturases can be heterologous expressed in Lactococcus lactis to produce the helpful fatty acids.
%K stearoyl-CoA desaturase-1
%K Lactococcus lactis
%K fatty acid<br>硬脂酰-CoA脱氢酶
%K 乳酸乳球菌
%K 脂肪酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=22653CDAF59BBDC1CE1704D73C301504&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=9CF7A0430CBB2DFD&sid=2D52C01434B71375&eid=87EA718095CEFCE5&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0