%0 Journal Article
%T Rapid genetic characterization of a novel Enterobacteria phage and determination of its host recognizing genes<br>一株新分离的肠杆菌噬菌体的快速遗传鉴定及其宿主识别基因的快速克隆
%A Huanhuan Jiang
%A Sheng Wang
%A Cun Li
%A Dabin Liu
%A Changming Yu
%A Xiaoping An
%A Zhiqiang Mi
%A Jiankui Chen
%A Yigang Tong
%A <br>姜焕焕
%A 王盛
%A 李存
%A 刘大斌
%A 于长明
%A 安小平
%A 米志强
%A 陈建魁
%A 童贻刚
%J 生物工程学报
%D 2011
%I 
%X We isolated a novel Enterobacteria phage IME08 from hospital sewage, then confirmed it was a double-stranded DNA phage by digesting its genetic material with DNase I, RNase A and several restriction endonucleases respectively. BLAST results of random fragments generated by a random PCR cloning method revealed that it belonged to T4-like virus. We subsequently determined the host recognizing genes (g37 and g38) sequence with a PCR-based "genome jumping" protocol based on highly conserved region at 5' terminus of g37 from four other T4-like Bacteriophages (T4, JS98, T2 and K3). These molecular biological methods enabled us to readily characterize the bacteriophage and efficiently determine the sequence of the genes of interest based on very limited conserved sequence information.
%K Enterobacteria phage
%K T4-like virus
%K PCR
%K genome jumping
%K host recognizing gene<br>肠杆菌噬菌体，T4噬菌体属，PCR，基因组跳跃，宿主识别基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=9880CD65C317F63D8B58F91069924B08&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=B31275AF3241DB2D&sid=BEBF2238C7F1C1F1&eid=38DB5AF4AD8FDCCA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0