%0 Journal Article
%T Mechanism of DNA transformation based on mineral nanofibers and method improvement<br>基于矿石纳米材料的DNA转化的机理初探及方法改进
%A Haidong Tan
%A Lei Wang
%A Jintao Lin
%A Zongbao
%A <br>谭海东
%A 王磊
%A 林金涛
%A 赵宗保
%J 生物工程学报
%D 2010
%I 
%X Sepiolite?an inexpensive, resourceful, fibrous yet inoffensive mineral?made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competitive test, we proposed the different transforming mechanisms from previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4°C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.
%K mineral nanofibers
%K sepiolite
%K DNA transformation
%K mechanism for transforming<br>矿石纳米纤维材料，海泡石，DNA转化，转化机制
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=02776F634F20A13B05357323FACB1037&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=F3090AE9B60B7ED1&sid=F2D4C4F6570F167A&eid=7A60741D2B519BE0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0