%0 Journal Article
%T The Expression, Purification and Activity Analysis of the Rice Chitinase Gene in Escherichia coli<br>水稻几丁质酶基因在大肠杆菌中的表达、纯化与活性分析
%A Aikui Chen
%A Lujun Yu
%A Jianming Fan
%A Dongru Feng
%A Jinfa Wang
%A <br>陈爱葵
%A 俞陆军
%A 樊剑鸣
%A 冯冬茹
%A 王金发
%J 生物工程学报
%D 2008
%I 
%X The cloned cDNA sequence of rice (Oryza sativa L. Cpslo17) chitinase gene Oschi was cloned, (which was registered in GenBank, the accession number: EU045451) ligated with the expression vector pGEX-4T-1, and transformed into E. coli BL21(DE3). The expression of Oschi was induced by IPTG, and the conditions were optimized. After purification the in vitro activity of Oschi chitinase was analyzed, and the results indicated that it could efficiently degrade chitin.
%K rice
%K chitinase
%K gene
%K pGEX-4T-1<br>水稻
%K 几丁质酶
%K 基因
%K pGEX-4T-1
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=7B87EAD4ADB8C3FE850B7E46C8BED174&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=847B14427F4BF76A&eid=C29816B2656377A7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=11