%0 Journal Article
%T Expression and Identification of PEBP-like Gene from Saussurea involucrate Kar.er kir in Escherichia coli<br>雪莲类PEBP基因在大肠杆菌中的表达及鉴定
%A Wang Zhifang
%A Zhang Xuejun
%A Wang Bo
%A Zhou Xiaoyun
%A Ai Xiulian
%A <br>王志方
%A 张学军
%A 王博
%A 周小云
%A 艾秀莲
%J 生物工程学报
%D 2008
%I 
%X This assay was designed to construct the prokaryotic expression vector, investigate the expression of PEBP-like in Escherichia coli and purify its product. The PEBP gene was inserted into the vector pET30a (+). The recombinant vector was transferred into E. coli BL21 (DE3)and induced the expression of protein by low concentration of IPTG and low temperature overnight. After purification, the supernatants were analyzed by SDS-PAGE and the results were identified by Western blotting. After IPTG induction, a new anticipating fusion protein of 28 kD appeared as an expected size, and its product was 26.8% in total protein, the fusion protein was positive by Western blotting. The prokaryotic expression system of PEBP-like is successfully constructed. It lays the foundation for the further application study on the antifreeze characters of the PEBP.
%K PEBP
%K prokaryotic expression
%K cold-induced
%K Saussurea involucrate Kar
%K er kir<br>磷脂酰乙醇胺结合蛋白
%K 原核表达
%K 低温诱导
%K 雪莲
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=248709B6E44AABC2B773D705A43DD7E1&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=9CF7A0430CBB2DFD&sid=4BFBB19F1A63A4F0&eid=1396DEC9DD671DC4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15