%0 Journal Article
%T Molecular and biochemical analysis of two genes encoding dehydroascorbate reductase in common wheat<br>普通小麦中双脱氢抗坏血酸还原酶(TaDHAR)基因的克隆与生化特性分析
%A Chunmei Yu
%A Yanping Yang
%A Xinyan Liu
%A Rong Zhou
%A Liang Hu
%A He Wei
%A Shengjie Ding
%A Daowen Wang
%A <br>余春梅
%A 杨艳萍
%A 刘鑫燕
%A 周蓉
%A 华梁
%A 魏贺
%A 丁胜杰
%A 王道文
%J 生物工程学报
%D 2009
%I 
%X Dehydroascorbate reductase (DHAR) plays an important role in the recycling of ascorbic acid. In this work, we isolated the full length cDNA clones of two different DHAR genes (tentatively named as TaDHAR1 and TaDHAR2, respectively) from common wheat. Semi-quantitative PCR experiments showed that TaDHAR1 and TaDHAR2 were transcribed in many vegetative and reproductive organs examined in this work. Transient expression analysis using wheat protoplasts indicated that the protein products of TaDHAR1 and TaDHAR2...
%K common wheat
%K dehydroascorbate reductase
%K ascorbic acid
%K dehydroascorbate
%K expression pattern
%K enzyme activity<br>普通小麦
%K 双脱氢抗坏血酸还原酶
%K 抗坏血酸
%K 双脱氢抗坏血酸
%K 表达模式
%K 酶活
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=93D6CA75C22909B33442C66634F7ABE8&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=F3090AE9B60B7ED1&sid=6E73C7EB16CBAB68&eid=1254F6F9A8625D48&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0