%0 Journal Article
%T Optimized expression of the L1 protein of human papillomavirus in Hansenula polymorpha<br>人乳头瘤病毒16亚型L1蛋白在多形汉逊酵母中的优化表达
%A Weiwei Li
%A Xiuping He
%A Xuena Guo
%A Zhenying Zhang
%A Borun Zhang
%A <br>李巍巍
%A 何秀萍
%A 郭雪娜
%A 张振颖
%A 张博润
%J 生物工程学报
%D 2009
%I 
%X The heterologously expressed L1 protein of human papilomavirus 16 can assembly into virus-like particles (VLPs), which has been used as prophylactic vaccine for cervical carcinoma. To express L1 protein in Hansenula polymorpha, we analyzed the codon usage of the native gene of L1 protein and redesigned the encoding sequence according to the codon bias of H. polymorpha. We used assembly PCR to synthesize the native gene HPV16L1-N and the codon optimized gene HPV16L1. The synthesized genes were cloned into pM...
%K human papilomavirus
%K 16 L1 protein
%K Hansenula polymorpha
%K optimized expression<br>人乳头瘤病毒16亚型
%K 衣壳蛋白L1
%K 多形汉逊酵母
%K 优化表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=75BDD1B352CDBD2E00857FB475141096&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=F3090AE9B60B7ED1&sid=CA590345B99F3387&eid=777A6C995272142B&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0