%0 Journal Article
%T Screening proteins interacting with infectious bursa disease virus Gt VP2 from cDNA library of B lymphoid cells of the bursa of fabricius<br>鸡法氏囊B淋巴细胞中与鸡传染性法氏囊病病毒VP2相互作用蛋白筛选
%A Yulong Gao
%A Fenfen Sun
%A Lei Hou
%A Honglei Gao
%A Xiaole Qi
%A Di Liu
%A Yuping Hua
%A Xiaomei Wang
%A <br>高玉龙
%A 孙芬芬
%A 侯磊
%A 高宏雷
%A 祁小乐
%A 刘娣
%A 华育平
%A 王笑梅
%J 生物工程学报
%D 2011
%I 
%X To screen the interactive proteins with IBDV Gt VP2 protein from cDNA library of B Lymphoid cells of the bursa of fabricius. The expression cDNA library plasmids was transformed to the yeast competent cells, which have the bait plasmid-Gt VP2. After testing for growth in synthetic complete medium lacking histidine and uracil and for production of b-galactosidase (X-gal), we obtained 16 positive clones. We searched the gene sequences of positive clones in the NCBI website. The blast results showed that five positive clones were the gallus sequences. They were Gallus gallus breed mitochondrial DNA, O_GlcNAc transferase, Tumor protein p53 binding protein, Stathmin and Chondroitin sulfate GalNAcT-2, respectively. This study is helpful for the further identifying the receptors of IBDV in B Lymphoid cells of the bursa of fabricius.
%K infectious bursa disease virus (IBDV)
%K B Lymphoid cells
%K cDNA expression library
%K protein-protein interactions
%K yeast two-hybrid system<br>传染性法氏囊病病毒，B淋巴细胞，cDNA表达文库，蛋白相互作用，酵母双杂交
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A1943F2FA0FBDE1F166CE74EF274A4C9&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=B4F9D541F855CF96&eid=90075EB19043D533&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=17