%0 Journal Article
%T Optimization of plant des-pGlu1-Brazzein gene according to yeasty biased codons and its expression in Pichia pastoris<br>植物甜蛋白des-pGlu1-Brazzein的密码子优化及其在毕氏酵母中的表达
%A Li Chunli
%A Han Lu
%A Zheng Zhenyu
%A Zhao Weidong
%A <br>Li C
%A Han L
%A Zheng Z
%A Zhao W
%J 生物工程学报
%D 2011
%I 
%X According to the amino acid sequence of des-pGlu1-Brazzein, 4 pairs of oligonucleotide with cosmic site were synthesized by using yeasty biased codons. After linkage and PCR, the 179 bp code area of des-pGlu1-Brazzein was obtained and inserted into pPIC9K, which resulted in the recombinant expression vector pPIC9K-Bra. By digestion with Sal I, the lined pPIC9K-Bra was transformed into Pichia pastoris GS115 by electric shock. The results of expression indicted that the secreted target protein accounted for 51.6% of total protein in the supernatant and showed biological activity after purification.
%K des-pGlu1-Brazzein
%K Pichia pastoris
%K expression
%K biological activity<br>甜蛋白，毕氏酵母，表达，活性检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=20E65ECF19352841061B4118FA05EE1F&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=5D311CA918CA9A03&sid=E2D70FDCD65737D1&eid=4E3541241257D204&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=11