%0 Journal Article
%T Establishment of Human IFN-g in vitro Release Assay and Its Application in Tuberculosis Diagnosis<br>人IFN-g体外释放检测法的建立及其在结核病诊断中的应用
%A Yingyu Chen
%A Quantao Deng
%A Zhihua Zhan
%A Aizhen Guo
%A Jie Xiang
%A Jun Chen
%A Jinhai Zhou
%A Qinzhi Zeng
%A Yixiang Du
%A Wu Wei
%A Qingwei Tong
%A Yanjie Chao
%A Youji Kuang
%A Huanchun Chen
%A <br>陈颖钰
%A 邓铨涛
%A 詹枝华
%A 郭爱珍
%A 项杰
%A 陈军
%A 周金海
%A 曾庆志
%A 杜义祥
%A 魏武
%A 童庆伟
%A 晁彦杰
%A 匡有吉
%A 陈焕春
%J 生物工程学报
%D 2008
%I 
%X This study aimed to establish human IFN-g (hIFN-g) in vitro release assay and to apply it in diagnosis of human tuberculosis. Human IFN-g gene was cloned and expressed in Escerichia coli. The recombinant hIFN-g was purified and used as immunogen to immunize mice and rabbits respectively. Monoclonal and polyclonal antibodies were respectively developed and a sandwich ELISA was established. The heparized whole blood from 111 active tuberculosis patients and 292 clinical healthy controls were collected. The blood was stimulated with tuberculosis specific fused antigen ESAT-6/CFP-10 and the plasma was collected for IFN-g detection. The sensitivity for tuberculosis diagnosis was 95.5%, whereas the positive detection rate for the healthy controls was 16.7%. There was a significant difference between the patients and healthy controls (P<0.01) indicating that this assay had a high sensitivity and specificity, and thus could be promising in tuberculosis diagnosis.
%K tuberculosis
%K IFN-g
%K diagnosis
%K ELISA
%K ESAT-6/CFP-10
%K monoclonal antibody<br>结核病
%K IFN-g
%K 诊断
%K ELISA
%K ESAT-6/CFP-10
%K 单克隆抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5461ECC6A47847E0332DB7A1C499EE17&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=9CF7A0430CBB2DFD&sid=4C9B788AD3F48EA4&eid=C48302AEE9AFB6AE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0