%0 Journal Article
%T Selection and Identification of Salt-tolerant Variants of Taraxacum officinale<br>药蒲公英(Taraxacum officinale Weber)耐1.5% NaCl变异体的筛选及特性分析
%A Xinguo Zhang
%A Yinxin Li
%A Hua Chen
%A Wuliang Shi
%A <br>张新果
%A 李银心
%A 陈华
%A 石武良
%J 生物工程学报
%D 2008
%I 
%X In order to obtain salt-tolerant variant plants of Dandelion (Taraxacum officinale Weber), the leaf discs were excised from 20 to 30-day old seedlings to produce callus, then the induced calli were transferred to selection mediums containing 1.5% NaCl. After regenerating and rooting, these salt-tolerant calli finally developed into 12 variant plantlets. Compared with the wild-type, these regenerated plants produced more trichomes on their leaves, and had larger leaves and shorter petioles. Additionally, the dumpy roots and an approximately 2-cm bract in middle parts of the floricanes were clearly observed in these salt-tolerant plants. By RAPD( Random Amplified Polymorphic DNA) and SDS-PAGE analysis, these salt-tolerant plants showed differences from the control at DNA and protein levels. With 1.5% NaCl treatment, the antioxidant enzyme activity, proline content, and flavonoid concentration were higher in these salt-tolerant plants, whereas maloaldehyde concentration was significantly lower. Salt-tolerant lines of T. officinale showed stronger anti-oxidative activity and higher flavonoid contents.
%K ROS scavenger
%K salt-tolerant variant
%K Taraxacum officinale
%K leaf explant<br>活性氧清除剂
%K 耐盐变异体
%K 药蒲公英
%K 叶片外植体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=BCA18A0E07B50E5BBB82E05ECA47D571&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=30897FA31CA3354D&eid=273ADA1BCEFE8C00&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=30