%0 Journal Article
%T Construction and Immunogenicity of a Genetic Engineered Strain Expressing Nontoxic ST1-LTB-a- b Fusion Protein Against Diarrhea of Piglet<br>表达ST1-LTB-a-b融合蛋白基因工程菌株的构建及其免疫原性分析
%A Jie Song
%A Mingming Qian
%A Jianing Bai
%A Baohua Zhao
%A <br>宋杰
%A 钱明明
%A 柏佳宁
%A 赵宝华
%J 生物工程学报
%D 2008
%I 
%X We constructed a recombinant strain BL21 (DE3) (pETST3LTBab) including ST1-LTB-a-b fusion gene via molecular technology. The SDS-PAGE and Western blotting indicated that the ST1-LTB-a-b fusion protein was highly expressed in Escherichia coli and the molecular weight of the fusion protein was about 110 kD. The recombinant strain was induced in different concentrations of lactose and different aeration rate. The optimal culture conditions in 20 L fermentor were 1% inoculation (V/V), initial aeration 5 L/min, 0.03 mol/L lactose addition 3 hours after inoculation, and increased the aeration to 12.5 L/min for the following 6 hours. The fusion protein was about 38.53% of total cellular protein. It was nontoxic, immunogenic and protective against enterotoxigenic E. coli and Clostridium perfringens infection The constructed recombinant strain BL21 (DE3) (pETST3LTBab) could serve as a candidate vaccine strain against diarrhea of piglet.
%K diarrhea of piglet
%K protective antigen
%K gene engineering strain
%K immunogenicity<br>仔猪腹泻
%K 保护性抗原
%K 基因工程菌株
%K 免疫原性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D7EC6002999A75447B982611B49128B9&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=5D311CA918CA9A03&sid=CEEFA682892739FB&eid=AFB21040E5F48417&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0