%0 Journal Article
%T Real time ex vivo detection and dynamic monitoring of in vivo expression of secreted luciferase gene injected by hydrodynamic method<br>体外实时动态监测水动力注射分泌型荧光素酶基因的表达
%A Wenhong Tian
%A Gang Wang
%A Shuntao Luo
%A Xiaoyan Dong
%A Xinyang Fu
%A Wenjie Tan
%A XiaobingWu
%A <br>田文洪
%A 王刚
%A 罗顺涛
%A 董小岩
%A 付昕阳
%A 谭文杰
%A 吴小兵
%J 生物工程学报
%D 2009
%I 
%X We chose Gaussia luciferase (Gluc), a secreted luciferase gene as reporter to real-time detect and dynamically monitor hydrodynamic injection gene expression. First, we constructed an expression vector pAAV2neo-Gluc. Then Huh7 and HepG2 cells were transfected with pAAV2neo-Gluc and the activity of Gluc in the supernatant and cell lysates were assayed. Results showed that the Gluc activity in the supernatant was about 100 higher than that in cell lysates, indicating the expressed Gluc existing mainly as a se...
%K hydrodynamic injection
%K gene expression
%K Gluc
%K monitoring
%K ex vivo<br>水动力注射
%K 基因表达
%K Gluc
%K 监测
%K ex
%K vivo
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=152C1765CAB03D1BC61A1D525E46C508&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=F3090AE9B60B7ED1&sid=857646F4875EA11E&eid=CDD86F65AA3A5D34&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0