%0 Journal Article
%T Stability of the hydrogenase from Tetraselmis subcordiformis and its preliminary purification<br>亚心形四爿藻氢酶的稳定性及初步纯化
%A 彦飞
%A 陈兆安
%A 曹旭鹏
%A 陆洪斌
%A 薛松
%A 张卫
%J 生物工程学报
%D 2010
%I 
%X Tetraselmis subcordiformis, a marine green alga, can produce hydrogen by photobiologically hydrolyzing seawater with hydrogenase. In this study, the preliminary purification of the enzyme was explored by ammonium sulfate precipitation, and the impact of sodium dithionite, b-mercaptoethanol and glycerol on the enzyme stability during the process was investigated. The experimental results illustrated that sodium dithionite provided significant protection on the hydrogenase by depleting oxygen, while glycerol, a protectant against the structure instability of the enzyme, also presented protection. Crude enzyme with specific activity of 0.557 U/mg protein was extracted using 60%?70% saturated ammonium sulfate solution supplemented with 200 mmol/L sodium dithionite and 5% glycerol, and the hydrogenase recovery yield was about 30%.
%K Tetraselmis subcordiformis
%K hydrogenase stability
%K ammonium sulfate precipitation<br>亚心形四爿藻，氢酶稳定性，硫酸铵分级沉淀
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=098116DE9BC9135F1164894D60EB8B87&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=DF92D298D3FF1E6E&sid=A8809BCBCBE59B72&eid=82BCA4C44409DD5C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0