%0 Journal Article
%T Subcellular localization and resistance to Botrytis cinerea of a new type lipid transfer protein AtDHyPRP1 from Arabidopsis thaliana<br>拟南芥新型脂转移蛋白AtDHyPRP1的亚细胞定位及其对灰霉菌的抗性
%A Chen Zhang
%A Lan Li
%A Ziqin Xu
%A <br>张晨
%A 李岚
%A 徐子勤
%J 生物工程学报
%D 2012
%I 
%X Genetic transformation was adopted to analyze the subcellular localization and the resistance to fungal pathogens of Arabidopsis lipid transfer protein AtDHyPRP1. The coding sequence of AtDHyPRP1 amplified by PCR from Ws ecotype was used to construct the plant binary expression vector pRI101-AN-AtDHyPRP1 and the fusion expression vector pCAMBIA1302- AtDHyPRP1-GFP. Transgenic tobacco and Arabidopsis plants were produced by leaf disc and floral dip protocols, respectively. AtDHyPRP1 could improve the resistance of tobacco to Botrytis cinerea remarkably and the infection sites on transgenic tobacco leaves accumulated large amounts of H2O2. Observation under laser scanning confocal microscope showed that AtDHyPRP1 was localized to cell surface. It suggested that AtDHyPRP1 might play special function after secretion to outside of the cell and was involved in plant defense system against pathogens.
%K AtDHyPRP1
%K Nicotiana tabacum qinyan 95
%K Arabidopsis thaliana
%K Botrytis cinerea Pers
%K ex of Garlic Sprout
%K DAB staining
%K trypan blue staining
%K subcellular localization<br>AtDHyPRP1，秦烟95，拟南芥，蒜薹灰霉菌，DAB染色，台酚兰染色，亚细胞定位
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=13A5D5D02B8AA870FA1FEEFF51EB126A&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=94C357A881DFC066&sid=3B2BF7AC5674E8E2&eid=EF9E84B2DA79FF23&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0