%0 Journal Article
%T High-level Production of Alkaline Polygalacturonate Lyase in Recombinant Pichia pastoris<br>重组毕赤酵母高密度发酵生产碱性果胶酶的策略
%A Yun Wang
%A Zhaozhe Hu
%A Liming Liu
%A Zhaohui Zhang
%A Guocheng Du
%A Jian Chen
%A <br>王芸
%A 华兆哲
%A 刘立明
%A 张朝晖
%A 堵国成
%A 陈坚
%J 生物工程学报
%D 2008
%I 
%X In order to increase the production of alkaline polygalacturonate lyase (PGL) by recombinant Pichia pastoris GS115, the effect of cell and methanol concentration on the PGL production was carefully investigated by single factor experiment. The optimum conditions were listed as follows: the cell concentration 122 g/L, the methanol concentration 20 g/L, and the ratio of methanol and cell concentration 0.16~0.20 g/g (methanol/cell). With the glycerol and methanol feeding strategies, the ratio of methanol and cell concentration could be controlled at the range of 0.171 to 0.195 g/g. And the highest PGL activity (430 u/mL) and highest PGL productivity (4.34 u/mL/h) were achieved.
%K Pichia pastoris
%K alkaline polygalacturonate lyase
%K ratio of methanol and cell concentration
%K feeding strategy<br>重组毕赤酵母
%K 碱性果胶酶
%K 甲醇与菌体比例
%K 流加策略
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4C544F2F3203464DE885EEF10BFEB9DC&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=20C9FB8C7B4A22AD&eid=46C2A519EDDA03DD&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=13