%0 Journal Article
%T Effects of trxS gene on protein degradation in germinating barley seeds<br>trxS基因对大麦发芽籽粒中蛋白质降解的影响
%A Li Wei
%A Weiwei Kong
%A Jun Yin
%A Weijie Wang
%A <br>卫丽
%A 孔维威
%A 尹钧
%A 王伟杰
%J 生物工程学报
%D 2009
%I 
%X We assessed the effects of trxS gene on changes of proteinase activity, contents of different protein fractions and SDS-PAGE profiles in germinating seeds of contrasting transgenic and nontransgenic barley variety. Proteinase activity was enhanced by 70.28% in transgenic than nontransgenic barley seeds, whereas contents of albumin, globulin, hordein and glutelin in transgenic seeds were 3.68%, 23.52%, 31.37%, and 21.04%, lower than those in nontransgenic seeds. Degradation rates of hordein and glutelin in transgenic seeds were faster than those in nontransgenic seedlings as indicated by the SDS-PAGE profiles. Our data imply that the transformation of trxS gene could promote the degradation of protein, providing theoretic basis for the use of trxS gene and barley quality breeding.
%K SDS-PAGE
%K trxS gene
%K proteniase
%K protein content
%K SDS-PAGE<br>trxS基因
%K 蛋白质
%K 蛋白酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4E7F6646E56624F324660EFFC7647651&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=B5A8A26068AC6AD1&eid=E4E9596BEA192161&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=18