%0 Journal Article
%T Cloning, expression and characterization of gene encoding human stem cell growth factor-alpha and its synergetic effect with rhGM-CSF on proliferation of human umbilical cord mesenchymal stem cells<br>人干细胞生长因子-α基因的克隆、表达及其协同rhGM-CSF对人脐带间充质干细胞的增殖作用
%A Xinlei Peng
%A Yanyan M
%A Jing Rong
%A Zhenling Zhao
%A Bo Han
%A Wei Chen
%A Yangfei Xiang
%A Qiuying Liu
%A Yifei Wang
%A Zhe Ren
%A Xiangrong Zhou
%A Haijia Chen
%A <br>彭鑫磊
%A 马岩岩
%A 荣靖
%A 赵振岭
%A 韩波
%A 陈伟
%A 向阳飞
%A 刘秋英
%A 王一飞
%A 任哲
%A 周向荣
%A 陈海佳
%J 生物工程学报
%D 2011
%I 
%X To investigate the effect of hSCGF-alpha on human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs), we obtained hSCGF-alpha using genetic engineering, hSCGF-alpha gene was amplified from hUCMSCs cDNA using two-step PCR and was inserted into pET-28a(+) plasmid vector. Induced by IPTG at 20 degrees Celsius for 24 h, the fusion protein expressed in E. coli BL21 (DE3) was mainly existing in soluble form. The recombinant hSCGF-a was purified using NI-NTA affinity chromatography and the purity was up to 90%. The colony forming test revealed that combined use hSCGF-alpha and rmGM-CSF (recombinant murine GM-colony stimulating factor, rmGM-CSF) had granulocyte/macrophage (GM) promoting effects on murine bone marrow GM progenitor. In addition, the results indicated that hSCGF-alpha and rhGM-CSF had stimulatory effect on hUCMSCs and their synergetic effect was the strongest.
%K stem cell growth factor
%K human umbilical cord mesenchymal stem cells
%K cloning
%K synergetic effect
%K colony<br>干细胞生长因子，人脐带间充质干细胞，克隆，协同作用，集落
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F437B1BF01E7F4864B07FF02E5493C5C&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=708DD6B15D2464E8&sid=0D3639620E137999&eid=937C5AD88B71B15A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=12