%0 Journal Article
%T Contamination mechanism and regeneration strategies of chromatographic resin in separation process for expression product from mammary gland bioreactor<br>乳腺生物反应器表达产物层析分离过程中介质的污染机理及其再生策略
%A Sun Xiyan
%A Zhang Yan
%A Li Yan
%A Luo Jian
%A Qin Peiyong
%A Su Zhiguo
%A <br>Sun X
%A Zhang Y
%A Li Y
%A Luo J
%A Qin P
%A Su Z
%J 生物工程学报
%D 2011
%I 
%X This study focused on the contamination mechanism and regeneration strategies of sulfopropyl ion exchange resin(SP Sepharose FF) during the separation of recombinant human lactoferrin from transgenic bovine milk.We analyzed primary constituents' contents in chromatorgraphic material and fractions.The results showed that the lipid in milk can clog the column or adhere to the resin through hydrophobic interaction,leading to an increase in column pressure.Some casein molecules were found to adsorb onto the res...
%K mammary gland bioreactor
%K recombinant bovine milk
%K ion exchange resin
%K contamination mechanism
%K regeneration strategies<br>乳腺生物反应器，重组牛乳，离子交换介质，污染机理，再生策略
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F437B1BF01E7F4865D65993A11E61928&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=708DD6B15D2464E8&sid=E521CC33DC22B84E&eid=ED2274D65CDCCA90&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=22