%0 Journal Article
%T Screening of food-grade microorganisms for biotransformation of D-tagatose and cloning and expression of L-arabinose isomerase<br>生物转化生产D-塔格糖的食品级菌种选育及L-阿拉伯糖异构酶的克隆表达
%A Yan Men
%A Yueming Zhu
%A Yuping Guan
%A Tongcun Zhang
%A Ken Izumori
%A Yuanxia Sun
%A <br>门燕
%A 朱玥明
%A 管于平
%A 张同存
%A 何森健
%A 孙媛霞
%J 生物工程学报
%D 2012
%I 
%X L-Arabinose isomerase (L-AI) is an intracellular enzyme that catalyzes the reversible isomerization of D-galactose and D-tagatose. Given the widespread use of D-tagatose in the food industry, food-grade microorganisms and the derivation of L-AI for the production of D-tagatose is gaining increased attention. In the current study, food-grade strains from different foods that can convert D-galactose to D-tagatose were screened. According to physiological, biochemical, and 16S rDNA gene analyses, the selected strain was found to share 99% identity with Pediococcus pentosaceus, and was named as Pediococcus pentosaceus PC-5. The araA gene encoding L-AI from Pediococcus pentosaceus PC-5 was cloned and overexpressed in E .coli BL21. The yield of D-tagatose using D-galactose as the substrate catalyzed by the crude enzyme in the presence of Mn2+ was found to be 33% at 40 °C.
%K L-arabinose isomerase
%K D-tagatose
%K food-grade microorganisms<br>L-阿拉伯糖异构酶，D-塔格糖，食品级微生物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=13A5D5D02B8AA870960677F8723BF39E&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=94C357A881DFC066&sid=106103EB0EA31435&eid=ED9DF3402785F68D&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0