%0 Journal Article
%T Direct secretory expression of active microbial transglutaminase in Pichia pastoris<br>利用毕赤酵母系统直接分泌表达具有活性的谷氨酰胺转胺酶
%A Pengfei Li
%A Hongbing Sun
%A Lijin You
%A Fuyu Gong
%A Zao Chen
%A Ailian Zhang
%A Taicheng Zhu
%A <br>李鹏飞
%A 孙红兵
%A 游丽金
%A 巩伏雨
%A 陈藻
%A 张爱联
%A 朱泰承
%J 生物工程学报
%D 2013
%I 
%X Direct secretory expression of active microbial transglutaminase (MTG) using heterologous hosts is a promising strategy although, while its production level still needs to be improved for industrial production. Pichia pastoris is one of the most efficient expression systems developed in recent years. In this study, secretory expression of active MTG was successfully achieved by co-expressing the pro sequence and mature MTG genes in P. pastoris. Furthermore, we optimized the copy number of pro/MTG expression cassettes and the fermentation conditions. MTG production level reached 7.3 U/mL in 1-liter fermentor through high density fermentation, providing the feasiblity for industrial scale preparation of MTG.
%K microbial transglutaminase
%K Pichia pastoris
%K copy number
%K co-expression
%K high density fermentation
%K pro sequence<br>谷氨酰胺转胺酶，毕赤酵母，拷贝数，共表达，高密度发酵，酶原序列
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2DB3B6D3544EDEBDF3C96018870CBDDD&yid=FF7AA908D58E97FA&vid=771469D9D58C34FF&iid=0B39A22176CE99FB&sid=F1A8654ADB4E656E&eid=847B14427F4BF76A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0