%0 Journal Article
%T Reuse of recombinant Escherichia coli to produce succinic acid by bioconversion<br>循环利用重组大肠杆菌细胞转化合成丁二酸
%A Bing Xu
%A Min Jiang
%A Jiangfeng M
%A Shuwen Liu
%A Guwei Hou
%A Shanshan Sui
%A <br>徐冰
%A 姜岷
%A 马江锋
%A 刘树文
%A 侯顾伟
%A 隋姗姗
%J 生物工程学报
%D 2010
%I 
%X The possibility of reusing Escherichia coli cells from the broth for succinic acid production was investigated. Using succinic acid yield and productivity as criterion, we investigated the effects of cell concentration, initial glucose concentration, different neutralizers on the bioconversion. The results revealed that E. coli could convert glucose to succinic acid in a water solution of glucose and a neutralizer. According to the results, the optimal condition was as follows: the cell concentration was 50 (OD600), glucose concentration was 40 g/L and neutralizer was MgCO3. Under the optimum conditions, we carried out the consecutive batch bioconversion in 7 L fermenter. Succinic acid yield reached 91% with the productivity of 3.22 g/(L·h) for the first conversion. For the second conversion, succinic acid yield reached 86% with productivity of 2.04 g/(L·h). Furthermore, we achieved a high mass yield above 83% with the productivity of 1.82 g/(L·h) for the third bioconversion.
%K succinic acid
%K consecutive batch bioconversion
%K Escherichia coli
%K two-stage fermentation<br>丁二酸，重复批次转化，大肠杆菌，两阶段发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5136ABC12240A329E953AB478F2A0390&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=708DD6B15D2464E8&sid=28652329520A4AA7&eid=F41021172F25CDE0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0