%0 Journal Article
%T Enhancement of 2-keto-L-gulonic acid production using three-stage pH control strategy<br>分阶段pH调控提高2-酮基-L-古龙酸生产
%A Jing Zhang
%A Jingwen Zhou
%A Liming Liu
%A Jie Liu
%A Kejie Chen
%A Guocheng Du
%A Jian Chen
%A <br>张静
%A 周景文
%A 刘立明
%A 刘杰
%A 陈克杰
%A 堵国成
%A 陈坚
%J 生物工程学报
%D 2010
%I 
%X The aim of this study was to improve the 2-keto-L-gulonic acid (2-KLG) production efficiency by Ketogulonicigenium vulgare and Bacillus megaterium by using multi-stage pH control strategy. The effect of pH on the cell growths and 2-KLG production showed that the optimum pH for K. vulgare and B. megaterium cell growth were 6.0 and 8.0, respectively, while the optimum pH for 2-KLG production was 7.0. Based on the above results, we developed a three-stage pH control strategy: the pH was kept at 8.0 during the first 8 h, then decreased to 6.0 for the following 12 h, and maintained at 7.0 to the end of fermentation. With this strategy, the titer, productivity of 2-KLG and L-sorbose consumption rate were achieved at 77.3 g/L, 1.38 g/(L·h) and 1.42 g/(L·h), respectively, which were 9.7%, 33.2% and 25.7% higher than the corresponding values of the single pH (pH 7.0) control model.
%K pH control strategy
%K Ketogulonicigenium vulgare
%K Bacillus megaterium<br>pH调控策略，酮古龙酸菌，巨大芽胞杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A5B770C4AC68DD7CB08739A2FF63F9AD&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=9CF7A0430CBB2DFD&sid=08DDB398556FE547&eid=0DC551EE075D9D73&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0