%0 Journal Article
%T Construction and Activity of Recombinant Adeno-associated Virus Expressing Vasostatin<br>表达血管抑制因子的腺相关病毒载体的构建及其体内外活性
%A Yong Diao
%A Jian M
%A Xinyan Li
%A Xueying Sun
%A Ruian Xu
%A <br>刁勇
%A 马健
%A 李欣燕
%A 孙学英
%A 许瑞安
%J 生物工程学报
%D 2008
%I 
%X Vasostatin, a 180-amino acid fragment from the N-terminal domain of calreticulin, is a potent endogenous angiogenesis inhibitor, which can inhibit the growth of many kinds of experimental tumor. But a recent report that vasostatin can enhance the malignant behavior of neuroendocrine tumor reminds us to be cautious to develop it as an anti-tumor medicine. VAS cDNA was cloned into pAAV-2 expression vector; recombinant virus rAAV-VAS was generated by a three plasmids, helper free packaging method. MS1 mouse pancreatic endothelial cell and human colon tumor HCT-116 cell were infected with rAAV-VAS. Transgene expression was analyzed by Western blotting analysis; cell proliferation was determined by MTT assay. The therapeutic potential of rAAV-VAS was evaluated in subcutaneous HCT-116 xenograft mouse model. rAAV-VAS inhibited the proliferation of MS1 but not HCT-116 cell. HCT-116 cell infected with rAAV-VAS secreted VAS protein into the supernatant effectively. The intratumoral delivery of rAAV-VAS inhibited the xenograft growth and microvessel density in tumors significantly. Our results show the effectiveness of rAAV-VAS as an angiogenesis inhibitor in suppressing tumor growth, validating the application of rAAV-VAS gene therapy in treatment against colon cancer.
%K vasostatin
%K colorectal neoplasms
%K angiogenesis
%K gene medicine
%K adeno-associated virus<br>血管抑制因子
%K 结肠肿瘤
%K 血管生成
%K 基因药物
%K 腺相关病毒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A8BAD20B8D787644ED4F4687E66B3DD8&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=708DD6B15D2464E8&sid=3D6B058F17849798&eid=D68286E0C08ACACF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=7